Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 PhenoSense GT® Plus Integrase
CPT:
87536
Synonyms
- HIV-1 Plasma Viremia
- HIV-1 RNA by Real-time PCR, Quantitative
- Reflex to PhenoSense GT® Plus Integrase
- Viral Load
Special Instructions
A separate frozen specimen is required for the PhenoSense GT® Plus Integrase portion of the test. A frozen sample is preferred for the viral load assay, but the sample is also stable at room temperature for one day or refrigerated for five days. Freeze the specimen for long-term storage.
Expected Turnaround Time
20 - 26 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
HIV-1 Lifecycle, Drugs, and Susceptibility Assays
Specimen Requirements
Specimen
Plasma, frozen
Volume
6 mL
Minimum Volume
4 mL (Note: This volume does not allow for repeat testing.)
Container
Ship in two or more screw-cap polypropylene frozen transport tubes
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to a screw-cap tube prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze. Separate frozen sample required for the PhenoSense GT® portion of the test. A frozen sample is preferred for the viral load assay, but the specimen is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™
Test Details
Use
Detect and quantitate HIV-1 in plasma with reflex to PhenoSense GT® Plus Integrase (at additional charge) if viral load is >500 copies/mL.
Limitations
Assay range: 20−10,000,000 copies/mL
Methodology
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550625 | RNA PCR(NonGraph)rfx/PSGT+IN | 20447-9 | 550445 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| 550625 | RNA PCR(NonGraph)rfx/PSGT+IN | 20447-9 | 550446 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| 550625 | RNA PCR(NonGraph)rfx/PSGT+IN | 20447-9 | 550626 | PhenoSenseGT(R) plus Integrase | N/A |
| Reflex Table for PhenoSenseGT(R) plus Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551930 | Reflex to PSGT plus Integrase | 551921 | PSGT plus Integrase PDF | 51969-4 | |
| Reflex Table for PhenoSenseGT(R) plus Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551930 | Reflex to PSGT plus Integrase | 551922 | PSGT+IN Net Assessment | 83326-9 | |
| Reflex Table for PhenoSenseGT(R) plus Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551930 | Reflex to PSGT plus Integrase | 551923 | PSGT+IN Phenotype Results | 49665-3 | |
| Reflex Table for PhenoSenseGT(R) plus Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551930 | Reflex to PSGT plus Integrase | 551924 | PSGT+IN Genotype Results | 80689-3 | |
| Reflex Table for PhenoSenseGT(R) plus Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551930 | Reflex to PSGT plus Integrase | 551925 | PSGT+IN Interpretation | 77202-0 | |
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