Acute Myelocytic Leukemia (AML) Profile, FISH
CPT:
Call client services.
Test Includes
FISH for acute myelocytic leukemia (AML), targeting rearrangement of PML-RARA, CBFB, RUNX1/RUNX1T1, KMT2A and numerical changes of 5q, and 7q
Special Instructions
Indicate pertinent clinical diagnosis and previous cytogenetic studies on the test request form.
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Blood, bone marrow, fixed-cell pellet from a cytogenetic analysis, or slides prepared from a cytogenetic analysis
Volume
10 mL blood (adult), 3 to 5 mL blood (pediatric), 1 to 3 mL bone marrow, one tube fixed-cell pellet, or ten slides
Minimum Volume
1 mL blood (adult/pediatric), 1 mL bone marrow, or six slides
Container
Green-top (heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Broken Vacutainer®; broken or dirty slides; excessive cellular debris or stained slides; frozen specimen; decalcified tissue
Test Details
Use
Diagnostic and prognostic test for acute myeloid leukemia; detection rate is 80% of the known chromosome causes of AML.
Limitations
Molecular mutations or chromosomal alterations not targeted by the probes included in this profile will not be detected.
Methodology
Fluorescence in situ hybridization (FISH)
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510336 | AML FISH Panel | 510709 | Specimen Type | 31208-2 | ||
| 510336 | AML FISH Panel | 510687 | Cells Counted | 62361-1 | ||
| 510336 | AML FISH Panel | 510688 | Cells Analyzed | 62360-3 | ||
| 510336 | AML FISH Panel | 510689 | FISH Result | 48674-6 | ||
| 510336 | AML FISH Panel | 510328 | Interpretation | 50398-7 | ||
| 510336 | AML FISH Panel | 510329 | Director Review: | 48672-0 | ||
| 510336 | AML FISH Panel | 512123 | 51967-8 |
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CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf