China Bioanalysis Forum 2024 -- Quantitation of total levels of monoclonal antibody (mAb) biotherapeutics in serum using ELISA may be hindered by soluble targets. Interfering molecules can form complexes with the analyte and inhibit the specific binding characteristics used for either capture or detection of the analyte in an assay. Based on Bead-Extraction and Heat-Dissociation (BEHD) which uses heat pre-treatment to minimize drug interference in ADA assay when the drug with lower melting temperature than full 150-kDa antibody (Xu et al. Immunological Methods, 2018), we hypothesized that target interference in PK assay can be dissociated or removed by sample heat pre-treatment. 

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