Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical)
CPT:
87536
Synonyms
- HIV-1 Plasma Viremia
- HIV-1 RNA by Real-time PCR, Quantitative
Special Instructions
This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550420.
Expected Turnaround Time
3 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
Human Immunodeficiency Virus 1/O/2 Cascade: Fourth-generation Antigen/Antibody Detection
Specimen Requirements
Specimen
Plasma
Volume
3.5 mL
Minimum Volume
1.1 mL (Note: This volume does not allow for repeat testing.)
Container
Lavender-top (EDTA) tube or plasma preparation tube (PPT™)
Collection
Centrifuge sample within 24 hours of collection. Transfer plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.
Storage Instructions
Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for six days. Stable frozen for six weeks.
Causes for Rejection
Incorrect anticoagulant; PPT™ frozen and/or not centrifuged
Test Details
Use
Detect and quantitate HIV-1 in plasma
Limitations
Assay range: 20−10,000,000 copies/mL
Methodology
Cobas® HIV
References
Wirden M, Larrouy L, Mahjoub N, et al. Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load. J Clin Virol. 2017 Nov;96:49-53.28950186
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550430 | RNA, Real Time PCR (Non-Graph) | 20447-9 | 550413 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| 550430 | RNA, Real Time PCR (Non-Graph) | 20447-9 | 550414 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
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