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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical)

CPT 87536
Synonyms
  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time PCR, Quantitative

Test Details

Methodology

Cobas® HIV

Result Turnaround Time

3 - 5 days

Use

Detect and quantitate HIV-1 in plasma

Special Instructions

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, use test 550430.

Specimen Requirements

Limitations

Assay range: 20−10,000,000 copies/mL

References

Wirden M, Larrouy L, Mahjoub N, et al. Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load. J Clin Virol. 2017 Nov;96:49-53.28950186

Specimen

Plasma

Volume

3.5 mL

Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)

Container

Lavender-top (EDTA) tube or plasma preparation tube (PPT™)

Storage Instructions

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for six days. Stable frozen for six weeks.

Causes for Rejection

Incorrect anticoagulant; PPT™ frozen and/or not centrifuged

Collection Instructions

Centrifuge sample within 24 hours of collection. Transfer plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550420 RNA, Real Time PCR (Graph) 20447-9 550413 HIV-1 RNA by PCR copies/mL 20447-9
550420 RNA, Real Time PCR (Graph) 20447-9 550414 log10 HIV-1 RNA log10copy/mL 29541-0
550420 RNA, Real Time PCR (Graph) 20447-9 511958 PDF 80563-0
Order Code550420
Order Code NameRNA, Real Time PCR (Graph)
Order Loinc20447-9
Result Code550413
Result Code NameHIV-1 RNA by PCR
UofMcopies/mL
Result LOINC20447-9
Order Code550420
Order Code NameRNA, Real Time PCR (Graph)
Order Loinc20447-9
Result Code550414
Result Code Namelog10 HIV-1 RNA
UofMlog10copy/mL
Result LOINC29541-0
Order Code550420
Order Code NameRNA, Real Time PCR (Graph)
Order Loinc20447-9
Result Code511958
Result Code NamePDF
UofM
Result LOINC80563-0