Human Immunodeficiency Virus 1 (HIV-1) GenoSure® Integrase
CPT:
Call 800-222-7566, ext 6-8400, for coding.
Synonyms
- HIV-1 Genotype
- Resistance Testing
- Retroviral Genotype
Special Instructions
Submit a separate frozen specimen for each test requested.
Expected Turnaround Time
9 - 15 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
Specimen Requirements
Specimen
Plasma, frozen
Volume
5 mL
Container
Lavender-top (EDTA) or plasma preparation tube (PPT™)
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Causes for Rejection
Use of green-top (heparin) tube; hemolysis; PPT™ not centrifuged; specimen at room temperature for more than 24 hours
Test Details
Use
Detect resistance of HIV-1 to antiretroviral drugs only in the integrase class.
Limitations
This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.
Methodology
Polymerase chain reaction (PCR) amplification and DNA sequencing
References
Cooper DA, Steigbigel RT, Gatell, JM, et al. Subgroup and resistance analyses of raltegravir for resistant HIV-1 infection. N Engl J Med. 2008 Jul 24; 359(4):355-365. 18650513
Evering T, Markowitz M. HIV integrase inhibitors.PRN Notebook. 2008; 13:1-9.
Hatano H, Lampiris H, Gupta S, et al. Long-term evolution of integrase resistance during failure of integrase inhibitor-based ART. Abstract 621, presented at 16th Conference on Retroviruses and Opportunistic Infections (CROI), February 2009, Montreal, Canada.
Malet I, Delelis O, Valantin MA, et al. Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro. Antimicrob Agents Chemother. 2008 Apr; 52(4):1351-1358. 18227187
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551871 | GenoSure(R) Integrase | 61199-6 | 551932 | 11502-2 | ||
| 551871 | GenoSure(R) Integrase | 61199-6 | 551933 | HIV GenoSure(R) Integrase | 93047-9 | |
| 551871 | GenoSure(R) Integrase | 61199-6 | 551934 | HIV GenoSure(R) Integrase | N/A |
| Reflex Table for HIV GenoSure(R) Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551319 | Quant, RNA PCR | 550415 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| Reflex Table for HIV GenoSure(R) Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551319 | Quant, RNA PCR | 550416 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| Reflex Table for HIV GenoSure(R) Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551913 | HIV GenoSure(R) Integrase | 551913 | HIV GenoSure(R) Integrase | N/A | |
| Reflex Table for HIV GenoSure(R) Integrase | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551914 | HIV GenoSure(R) Integrase I... | 551914 | HIV GenoSure(R) Integrase I... | N/A | |
© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.
CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf