Human Immunodeficiency Virus 1 (HIV-1) GenoSure PRIme®
CPT:
Call 800-222-7566, ext 6-8400, for coding.
Synonyms
- GenoSure PRime®
- HIV-1 Genotype
- POL Genotype
- Resistance Testing
- Retroviral Genotype
Special Instructions
Submit a separate frozen specimen for each test requested.
Expected Turnaround Time
11 - 14 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
A Comprehensive Resistance Profile in a Single Test
Specimen Requirements
Specimen
Plasma, frozen
Volume
5 mL
Container
Lavender-top (EDTA) or plasma preparation tubes (PPT™)
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or Pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at - 20° C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze for shipment or long-term storage.
Stability Requirements
Temperature | Period |
|---|---|
Frozen | 2 years |
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles
Test Details
Use
Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors.
Limitations
This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.
Methodology
Polymerase chain reaction (PCR) amplification and DNA sequencing.
References
Boyd SD, Maldarelli F, Sereti I, et al. Transmitted raltegravir resistance in an HIV-1 CRF_AG-infected patient. Antivir Ther. 2011; 16(2):257-261. 21447876
Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.
Thompson MA, Aberg JA, Cahn P, et al. Antiretroviral treatment of adult HIV infection: 2010 recommendations of the International AIDS Society-USA panel. JAMA. 2010 Jul 21, 304(3):321-333. 20639566
Young B, Fransen S, Greenberg K, et al. Transmission of integrase strand-transfer inhibitor multidrug-resistant HIV-1: Case report and response to raltegravir-containing antiretroviral therapy. Antivir Ther. 2011; 16(2):253-256. 21447875
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551700 | GenoSure PRIme(R) | 80689-3 | 551703 | 51969-4 | ||
| 551700 | GenoSure PRIme(R) | 80689-3 | 551701 | HIV GenoSure PRIme(R) | 93047-9 | |
| 551700 | GenoSure PRIme(R) | 80689-3 | 551702 | HIV GenoSure PRIme(R) | 80689-3 | |
| 551700 | GenoSure PRIme(R) | 80689-3 | 551706 | HIV GenoSure PRIme(R) Interp | 62364-5 |
| Reflex Table for HIV GenoSure PRIme(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551704 | GenoSure PRIme(R) | 551704 | GenoSure PRIme(R) | N/A | |
| Reflex Table for HIV GenoSure PRIme(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550503 | Quant, RNA PCR | 550494 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| Reflex Table for HIV GenoSure PRIme(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550503 | Quant, RNA PCR | 550495 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| Reflex Table for HIV GenoSure PRIme(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551707 | GenoSure PRIme(R) Interp | 551707 | GenoSure PRIme(R) Interp | N/A | |
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