Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to PhenoSense Entry®

TEST: 550330
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CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time (RT) PCR, Quantitative
  • Reflex to PhenoSense Entry®
  • Viral Load

Expected Turnaround Time

5 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

    Specimen Requirements

    Specimen

    Plasma, frozen

    Volume

    6 mL (3 mL for viral quantitation and 3 mL for reflex to PhenoSense Entry®)

    Minimum Volume

    4 mL (Note: This volume does not allow for repeat testing.)

    Container

    Ship in two or more screw-cap polypropylene frozen transport tubes.

    Collection

    Collect specimen in lavender-top (EDTA) or PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to screw-cap tubes prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

    Storage Instructions

    Freeze. Separate frozen sample required for PhenoSense Entry® portion of the test. Frozen sample is preferred for the viral load assay but is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.

    Causes for Rejection

    Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™

    Test Details

    Use

    Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.

    Limitations

    Assay range: 20−10,000,000 copies/mL

    Methodology

    Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    550330 RNA, PCR (Graph) rfx/Entry 550445 HIV-1 RNA by PCR copies/mL 20447-9
    550330 RNA, PCR (Graph) rfx/Entry 550446 log10 HIV-1 RNA log10copy/mL 29541-0
    550330 RNA, PCR (Graph) rfx/Entry 550331 PhenoSense Entry (TM) N/A
    550330 RNA, PCR (Graph) rfx/Entry 511958 PDF 80563-0
    Reflex Table for PhenoSense Entry (TM)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551122 Reflex to PhenoSense Entry(R) 550247 PhenoSense Entry(R) PDF 51969-4
    Reflex Table for PhenoSense Entry (TM)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551122 Reflex to PhenoSense Entry(R) 550248 PhenoSense Entry(R) 82719-6
    Reflex Table for PhenoSense Entry (TM)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551122 Reflex to PhenoSense Entry(R) 550249 PhenoSense Entry(R) Interp. 77202-0

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    CPT Statement/Profile Statement

    The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf