Test Details
Methodology
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0
Result Turnaround Time
5 - 7 days
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.
Specimen Requirements
Limitations
Assay range: 20−10,000,000 copies/mL
Specimen
Plasma, frozen
Volume
6 mL (3 mL for viral quantitation and 3 mL for reflex to PhenoSense Entry®)
Minimum Volume
4 mL (Note: This volume does not allow for repeat testing.)
Container
Ship in two or more screw-cap polypropylene frozen transport tubes.
Storage Instructions
Freeze. Separate frozen sample required for PhenoSense Entry® portion of the test. Frozen sample is preferred for the viral load assay but is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™
Collection Instructions
Collect specimen in lavender-top (EDTA) or PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to screw-cap tubes prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550330 | RNA, PCR (Graph) rfx/Entry | 550445 | HIV-1 RNA by PCR | copies/mL | 20447-9 | |
| 550330 | RNA, PCR (Graph) rfx/Entry | 550446 | log10 HIV-1 RNA | log10copy/mL | 29541-0 | |
| 550330 | RNA, PCR (Graph) rfx/Entry | 550331 | PhenoSense Entry (TM) | N/A | ||
| 550330 | RNA, PCR (Graph) rfx/Entry | 511958 | 80563-0 | |||
| Order Code | 550330 | |||||
| Order Code Name | RNA, PCR (Graph) rfx/Entry | |||||
| Order Loinc | ||||||
| Result Code | 550445 | |||||
| Result Code Name | HIV-1 RNA by PCR | |||||
| UofM | copies/mL | |||||
| Result LOINC | 20447-9 | |||||
| Order Code | 550330 | |||||
| Order Code Name | RNA, PCR (Graph) rfx/Entry | |||||
| Order Loinc | ||||||
| Result Code | 550446 | |||||
| Result Code Name | log10 HIV-1 RNA | |||||
| UofM | log10copy/mL | |||||
| Result LOINC | 29541-0 | |||||
| Order Code | 550330 | |||||
| Order Code Name | RNA, PCR (Graph) rfx/Entry | |||||
| Order Loinc | ||||||
| Result Code | 550331 | |||||
| Result Code Name | PhenoSense Entry (TM) | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 550330 | |||||
| Order Code Name | RNA, PCR (Graph) rfx/Entry | |||||
| Order Loinc | ||||||
| Result Code | 511958 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 80563-0 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551122 | Reflex to PhenoSense Entry(R) | 550247 | PhenoSense Entry(R) PDF | 51969-4 | |
| Reflex 1 | ||||||
| Order Code | 551122 | |||||
| Order Name | Reflex to PhenoSense Entry(R) | |||||
| Result Code | 550247 | |||||
| Result Name | PhenoSense Entry(R) PDF | |||||
| UofM | ||||||
| Result LOINC | 51969-4 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551122 | Reflex to PhenoSense Entry(R) | 550248 | PhenoSense Entry(R) | 82719-6 | |
| Reflex 1 | ||||||
| Order Code | 551122 | |||||
| Order Name | Reflex to PhenoSense Entry(R) | |||||
| Result Code | 550248 | |||||
| Result Name | PhenoSense Entry(R) | |||||
| UofM | ||||||
| Result LOINC | 82719-6 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551122 | Reflex to PhenoSense Entry(R) | 550249 | PhenoSense Entry(R) Interp. | 77202-0 | |
| Reflex 1 | ||||||
| Order Code | 551122 | |||||
| Order Name | Reflex to PhenoSense Entry(R) | |||||
| Result Code | 550249 | |||||
| Result Name | PhenoSense Entry(R) Interp. | |||||
| UofM | ||||||
| Result LOINC | 77202-0 | |||||