Immunofixation (IFE), Serum
CPT:
82784(x3) (IgG, IgA, IgM); 86334 (immunofixation electrophoresis)
Synonyms
- Electrophoresis, Serum, IFE
- Immunoelectrophoresis, Serum
Test Includes
Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of monoclonal heavy- (IgG, IgA, IgM, IgD, IgE) and light-chain (kappa, Lambda) patterns, if present in the serum
Special Instructions
State patient's age and clinical diagnosis on the test request form.
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
For more information, please view the literature below.
Specimen Requirements
Specimen
Serum
Volume
2 mL
Minimum Volume
1 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Collection
Separate serum from red cells.
Storage Instructions
Room temperature
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Patient Preparation
Overnight fasting is preferred, but not required.
Causes for Rejection
Plasma sample
Test Details
Use
Detect and identify monoclonal immunoglobulin gammopathies
Methodology
Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); turbidimetric quantitation of IgA, IgG, and IgM
Reference Interval
Normal pattern
Additional Information
Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 001685 | Immunofixation, Serum | 34550-4 | 001795 | Immunofixation Result, Serum | 14895-7 | |
| 001685 | Immunofixation, Serum | 34550-4 | 001776 | Immunoglobulin G, Qn, Serum | mg/dL | 2465-3 |
| 001685 | Immunofixation, Serum | 34550-4 | 001784 | Immunoglobulin A, Qn, Serum | mg/dL | 2458-8 |
| 001685 | Immunofixation, Serum | 34550-4 | 001792 | Immunoglobulin M, Qn, Serum | mg/dL | 2472-9 |
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